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| Serum Levels of Interleukin-10 and Interferon Gamma Among Patients with Plasmodim Falciparum EL-DAMAZIEN - BLUE NILE STATE- SUDAN |
Ahmed Abdalfatah Ahmed Galander, Prof. Hamid Suliman Abdullah, Tayseer Elamin Mohamed Elfaki
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Abstract:
Abstract: Cytokines play an important role in human immune responses to malarial disease. However, the role of these mediators in disease pathogenesis, and the relationship between host protection and injury remains unclear. In malaria, blood concentrations of cytokines were influent by the infection, such as interleukin (IL-10) and interferon-? (IFN-?). In this study serum levels of interleukin-10 (IL-10) and interferon-? (IFN-?) were determined in 52 patients and 7 healthy volunteers as controls in El-Damazien, Blue Nile State- Sudan. Blood samples were collected from both patients and control candidates in (EDTA) containers for parasitological and immunological tests. Microscopic examination for Geimsa stained thick and thin blood films were used to detect the positive samples for Plasmodium falciparum. Enzyme linked Immunosorbant Assay (ELISA), was used for the determination of IL-10 and IFN-? levels. Serum levels of IL-10 and IFN-? were markedly elevated in patients with malaria (118.99 ± 62.21 ng/ml versus 12.08± 7.77 ng/ml in healthy controls; 40.45 ± 35.31 ng/ml versus 12.33 ± 5.00 ng/ml, respectively; mean ± SD). These results were found to be statistically significant (P= 0.007). Furthermore the levels of these cytokines had significant correlation within parasitemia (0.001 and 0.022 respectively). Thus suggest that stimulatory and inhibitory cytokines for macrophage activation and/or antibody production (i.e., TH1- and TH2-type immunoreaction, respectively) are coexpressed during acute P. falciparum infection and stress the multifactorial network between host and parasite in malaria immunology. Further studies are needed to examine whether serum concentrations of these cytokines also parallel their concentrations at the tissue sites of their production and action.
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