International Journal of Academic and Applied Research (IJAAR)   Year: 2019 | Volume: 3 | Issue: 8 | Page No.: 32-45 In Vivo Evaluation of Seed Dressing Fungicides against Karnal Bunt of Wheat Umer Zaib, Abdul Salam Mengal, Sohail Iqbal, Muhammad Hayat,Mueen Uddin, Abdul Hayee,Khalid Ahmed. Abstract: Disease management is a key component of high-yielding wheat production. Seed treatment fungicides form low cost crop insurance. In order to assess the efficacy of different fungicides as a seed dressing against karnal bunt of wheat caused by Tilletia indica, an in vivo study was conducted at the experimental field of Agriculture Research Institute Quetta during 2014. In vitro study was carried out to isolate seed borne fungi from wheat grains collected from Tandojam city. In vivo study was comprised of six fungicides treatments (T1 = Vibrax, T2 = Thiophenate, T3 = Definite, T4 = Protocol, T5 = Success and T6 = Control) which was tested against karnal bunt of wheat in randomized complete block design with three replication. The results showed that wheat grains cultured on potato dextrose medium indicated colonies of seven fungal genera including Atlernaria, Aspergillus, Fusarium, Rhizopus and Tilletia presenting twelve fungal species. Among them, the Aspergillus flavus was dominant with 18.8%occurrence. The in vivo study revealed that in comparison to control seed dressing with Vibrax and Thiophenate exhibited maximum germination percentage (97.73 and 98.00), less disease incidence (7.96%), less infected spikes and spikelets, 65.23 grains spike-1, tillers m-2 (413.67), biological yield (9114 kg ha-1), grain yield (3851 kg ha-1), seed index (43.03 g) and harvest index (45.18%). However, untreated seed showed maximum number of 197 infected spikes m-2 treatment-1 and 20.67 spikelets spike-1 respectively. Among the five fungicides, Vibrax and Thiophenate showed higher suppressiveness as a seed dressing and improved germination percentage, yield and yield components of wheat with less disease incidence and no affected spikes and spikelets. So, it can be inferred from this study that seed borne disease like karnal bunt of wheat can be controlled or managed by using systemic fungicides as seed dressing.