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| Assessment of Serological Tests for Diagnosis of H. pylori Infection among Dyspeptic Patients, Gezira State, Sudan |
Yosria Mohammed Elsiddig, Romysaa Magdi Sameir, Hadia Eldaw Mohammed, Tassneem EmadEldein Alshaikh, Omer Hassen M. Arabi, Mohamed Ibrahim M. Malik, Khalid Abdelsamea Mohamedahmed
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Abstract:
: Background: H. pylori is one of the most common damaging human pathogen, it identifies as common agent of gastroenteritis infections causes more hard GIT symptoms and potential complications like stomach cancer. Prevalence of H. pylori in Africa was reported about 79.1%, most likely due to socioeconomic factors. Several techniques have been developed to give the more reliable results for patient’s administration and to insure that the organism was eliminated flowing treatment.
Objectives: Many laboratories in Gezira State, Sudan using serological tests to screen and diagnosis the H. pylori through antigen and anti H. pylori antibodies. There for this study was conducted in adult dyspeptic patients to evaluate ELISA and ICT in diagnosis of symptomatic H. pylori infections and comparing with PCR for detection of 16s rRNA gene.
Method: Descriptive cross-sectional study was conducted on selected 102 adult dyspeptic patients their age ranged between (20 - 70 years) attending Gezira Center for G.I.T Endoscopy and Laparoscopic Surgery during 2016 - 2019. Stool, venous blood and Antrum biopsy samples were collected from each participants. ICT was used to screen the stool antigen and serum IgG antibody. ELISA technique was performed to investigate serum IgG and IgA. 16s rRNA gene was detected in antrum biopsy sample using PCR. The data of study group was collected by constructed questionnaire and analyzed by SPSS program ( V. 20).
Results: Epigastric pain (74.0%) was most common clinical finding among patients. According to the PCR method out of 102 samples 53 (51.9%) were positive for H. pylori and 49 (48.1%) were negative. 31 (30.4%) of patients were positive IgA by ELISA with sensitivity (66.8%), specificity (75.0%) and AUC (0.551). In contrast 71 (69.3%) patients were positive IgG by ELISA with sensitivity (69.8%), specificity (31.2%), and AUC (0.505). Furthermore 60 (58.8%) patients were positive ICT for serum IgG with sensitivity (45.3%), specificity (62.5%), and AUC (0.539). While 48 (62.34%) patients were positive ICT for stool Ag with sensitivity (79.2%), specificity (58%.6) and AUC (0.689).
Conclusion: Serological tests are simple, fast and easy technique but had many limitations for screening of H. pylori infection so the serological tests for diagnosis of H. pylori should be confirmed with other more diagnostic tests.
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