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| Phyto-Synthesis, Characterization and Evaluation of Antioxidant Potential of Iron Oxide Nanoparticles Using Lallemantia Royleana Seed Extract |
Sadia Noor, Samina Rashid Ahmed, Maria Anwar Khan
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Abstract:
In the present study a simple, cost effective and eco-friendly synthesis of iron oxide Nanoparticles (IONPs) has been carried out by the reduction of iron-chloride using ascorbic acid (AA) as reducing agent and Lallemantia royleana (LR) seed extract as stabilizing agent. The Fourier transform infrared spectroscopy (FTIR) analysis revealed the polysaccharide nature of LR/extract responsible for stabilization of iron oxide Nanoparticles. Optimization of different reaction parameters (pH, temperature, amount of AA) were carried out using conventional method. The optimum conditions were found to be temperature (50oC); amount of LR/extract (10mL) and amount of AA (1.0g). The synthesized IONPs were characterized by different analytical techniques; UV-visible spectroscopy, FTIR, Atomic absorption spectroscopy (AAS), Thermal gravimetric analysis (TGA) and Atomic force microscopy (AFM). The synthesis of IONPs was confirmed successfully by UV-visible spectroscopy which measured the characteristic band of IONPs at ~370 nm. FTIR showed the interaction between IONPs and different functional groups responsible for reduction and stabilization. Unreacted amount of iron oxide nanoparticles was measured by AAS and maximum yield was found to be 83.78% by varying LR amount. The topography and surface roughness of IONPs was confirmed by AFM. The antioxidant potential of synthesized IONPs was confirmed using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH), reducing power and hydroxyl radical scavenging assays which shows a concentration dependent effect on the antioxidant potential of IONPs.
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