Abstract:
Hydrogen cyanide (HCN) is a very common volatile toxic antimicrobial secondary metabolite produced by a number of microorganisms including plant growth promoting rhizobacteria (PGPR) that plays a major key role in plant pathogenic disease suppression through its effective toxicity to the phytopathogens and also serves as an important factor in regulation of phosphorous availability to plants as well as in weed control. A considerable numbers of bacteria produce hydrogen cyanide (HCN) as their volatile or exogenous metabolite secreted in microbial culture. This characteristic can be exploited as a substantial quality to fight against plant and crop pathogens as well as a potential weed growth controlling factor in agricultural fields.
Aiming the perspective mentioned above, the present work deals with the isolation, purification and identification of a purple non sulfur bacterium (PNSB), tentatively identified as Rubrivivax gelatinosus (strain RASN4) and evaluation of its potentiality for HCN production (in vitro) as a substantial phytopatho-remidiatory quality against a wide range of agricultural crop and plant pathogens and vis-à-vis as an effective measure to control the growth of the weeds in crop fields beside of its other plant growth promoting rhizobacterial (PGPR) features in order to formulate a potential microbial biofertilizer.
For this said purpose, this said purple non sulfur bacterium (PNSB) Rubrivivax gelatinosus (strain RASN4) was first screened qualitatively followed by its quantitative determination of bacterial HCN production (in vitro) in order to confirm this assessment. This is the first time report that the purple non sulfur bacterium (PNSB) Rubrivivax gelatinosus strain RASN4 can produce HCN. Quantitative study showed that the amount of HCN produced by R. gelatinosus (strain RASN4) attended its optimum level on the second day (131µg/ml) through regularly routine examination of the HCN estimation produced exogenously in culture medium up to 15th date of its experimental incubation
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