Abstract:
The study was conducted to evaluate factors to consider in measuring serum Interleukin (IL)-8 levels in Sudanese patients infected with S. mansoni. A cross-sectional study was conducted in New Halfa City-Eastern Sudan during the period from March to October 2011. The study was conducted on 575 subjects, 332 were males (45 were positive for S. mansoni and 287 were negative for S. mansoni) and 243 were females (65 were positive for S. mansoni and 178 were negative for S. mansoni). The age ranged between 4-85 years old, and the age was divided into 5 groups: 4-12, 13-19, 20-45, 46-60 and 61-85 years old. Faeces samples were collected from all the subjects included in the study and examined by using Kato-Katz technique. Also, serum samples were collected from all subjects and examined by using Human IL-8 ELISA Ready-SET-Go! (eBioscience) technique. The results showed that S. mansoni-infected individuals have no differences in their levels of IL-8 when was compared in two groups (infected and uninfected by using Mann-Whitney test), p=0.4331 and in three groups (infected, uninfected and previously infected by using Kruskal-Wallis test), p=0.0656. But, when `was compared infected with uninfected p=0.0139 and previously infected with uninfected p=0.0074. The results reflected that there was a statistically significant relationship between the mean of IL-8 and gender, age-groups, treatment of S.mansoni infection, Kato-Katz technique and intensity of S. mansoni infection and there was a statistically insignificant relationship between the mean of IL-8 and the tribes. The results revealed that the mean of serum IL-8 in S. mansoni infection reflected positive correlation with gender, treatment, Kato technique, intensity of infection, tribes and age groups.
|