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| Heat Resistance of Bacillus Species Isolated From Tsire-Suya |
Abideen Adeyinka Adekanmi, Olasupo Abdulhakeem Dapo, Adewale Saheed Adekanmi, Muraina Taoreed Adekunle
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Abstract:
Microorganisms involved during thermal processing have dual roles in industrial setting; most industrialist and food experts give attention to havoc associated with microbes without assessing and investigating beneficial metabolites linked to them. Most of heat resistance microorganisms are thermophiles and their ability to survive certain heat treatment defined their applications in industrial development. The objective of this study was to determine the thermal resistance characteristics of Bacillus species obtained from Tsire-suya. The heat resistance for the three Bacillus species was determined by heating the tubes containing nutrient broth and the culture of the isolates with combination of time and heating temperature at 40, 45, 50,55, and 60oC during 5, 10, 15 and 20 minutes in a water bath. A total number of ten isolates were obtained from Tsire-suya samples. The isolates were screened for heat resistance activity, 3 out of the 10 isolates survived heat treatment. Bacillus sterothemophilus F2n, Bacillus licheniformis F1t and Bacillus subtilis W4m were selected and considered for further study. The result showed that Bacillus stearothermophlius (F2n) had D-value of 14.58, 12.44, 15.20, 14.62, and 12.27 minutes at 40,45,50,55, and 60oC respectively while D-values of 15.38, 7.99, 10.59, 6.68, and 7.12 minutes for Bacillus subtilis (w4m) at 40 45, 50, 55, and 60oc whereas Bacillus licheniformis (F1t) had D-values of 16.03, 19.53, 20.93 9.17 and 8.71 minutes at 40, 45, 50, 55 and 60oC respectively. Z-values were 1000, 65.79, and 58.82oC, respectively, for Bacillus stearothermophilus F2n), Bacillus subtilis W4m), and Bacillus licheniformis F1t. This study demonstrated that thermally resistant bacteria isolated from Tsire-suya exhibit high heat resistance for a wide range of temperature.
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