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| Immunohistochemical Detection of Epstein Barr Viruse among Lymphoma Sudanese Patients |
Abu Elgasim Abass Awad Elkareem and Nariman Osman Mohammed Alhassan
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Abstract:
This is retrospective descriptive case study aimed to detect the presence of Epstein Barr virus in lymphoma patient's samples using immunohistochemical method. Sixty paraffin embedded blocks previously diagnosed as lymph node lesions were collected. Samples include 50 malignant tumors (11 Hodgkin lymphoma and 39 non Hodgkin lymphoma) and 10 samples were benign tumors. One section of 3µm thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (modified new indirect method) for detection of EBV. Data was collected from patient's files and the obtained results were analyzed using SPSS computer program. The patient's age ranged between 7month and 80 years with mean 40 years, most patients 33 (55%) were more than 40 years and the remaining 27 were less than 40 years representing (45%) patients were more than 40 years. The majority of patients were males and the male: female ratio was 2.3:1 representing 42(70%) males and the remaining 18(30%) were females. Immunohistochemical detection of EBV was revealed positive result in 3/50 samples and negative result in 47/50 in malignant samples while all benign tumors showed negative result for EBV, with insignificant statistical association between EBV expression and histopathology diagnosis (P=0.427). The study concludes that EBV is detected in few samples of lymphoma samples.
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