International Journal of Academic Pedagogical Research (IJAPR)   Year: 2021 | Volume: 5 | Issue: 11 | Page No.: 1-6 Comparative Estimation of Strychnine and Brucine in Ashodhit (Impure) and Shodhit (Pure) Kuchala (Strychnos Nux Vomica) By Hptlc Study. Kadale Jagruti , Dongre Sushma , Sagbhor Ravindra Abstract: Kuchala (Strychnos nux vomica) is the most important Upavisha which have wide therapeutic potential. Its main alkaloids Strychnine and Brucine are Neurotic Poison. So, it needs proper purification in order to pacify the toxic effect of these alkaloids. In present study, Shodhana (Detoxification) of Kuchala is done by 3 different methods. It is purified by using Kanji, Godugdha and Gomutra. After proper Shodhana got 62.5%, 70% and 65% yield of Shuddha kuchala. In present study, estimation of alkaloids in raw Kuchala Done by HPTLC study with respect to two markers i.e Strychnine and Brucine. HPTLC study shows that Strychnine % gets lower in Gomutra and Brucine % gets lower in Kanji. HPTLC profile of Raw and Shodhita Kuchala indicate that some peaks disappeared and some new peaks appeared after shodhana process. This disappearance and appearance of new peaks suggest the extraction of some components like Strychnine, Brucine and formation of some new compound during Shodhana process. In HPTLC study, highest reduction in the toxic alkaloids strychnine and Brucine contents in the Gomutra and Kanji respectively. This study shows that alkaloids present in the Kuchala are soluble in alkaline (Gomutra) and acidic (Kanji) media.