Abstract:
This is a descriptive retrospective hospital based study conducted in Khartoum state hospitals. The study aimed to detect the expression of Bcl-2 proteins in cervical tumors using immunohistochemical method.Forty paraffin embedded blocks previously diagnosed as cervical tumors were selected. Samples include 30 (75%) malignant tumors (28/30) samples were squamous cell carcinoma and (2/30) samples were adenocarcinoma and 10 (25%) samples were benign tumors.The patient's age ranged between 30 and 85 years with mean age of 55 years, most patients 26 (65%) were less than 70 years and the remaining 14 (35%) patients were more than 70 years.One section of 3 µm thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (modified new indirect method) for detection of Bcl-2. Data collected from patient's files and results obtained were analyzed using SPSS computer program.Immunohistochemical expression of Bcl-2 was revealed positive result in 12/30 samples and negative result in 18/30 in malignant samples while all benign tumors showed negative result for Bcl2, with significant statistical association between Bcl-2 expression and histopathology diagnosis (P=0.02).Out of 12 Bcl2 positive samples, 11 samples were squamous cell carcinoma and only one sample was adenocarcinoma with no statistical association between Bcl-2 expression and type of cancer (P=0.78).Comparison between the expression of Bcl-2 and grade of the cancer the positive result was detected in 2/4 samples of well differentiated tumors, 5/12 samples of moderately differentiated tumors and 4/12 samples of poorly differentiated tumors with no relation between histological grade of tumor and Bcl-2 expression (P=0.55).This study concludes that there is association between Bcl-2 expression and malignant tumors of cervix, with no association with both type of cancer and the histological grade of tumor.
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