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| The Role of Cytocentrifugation Liquid Based Cytology (CLBC) in Improving the Efficacy of FNA in the Detection of Cytological Changes in Breast Lumps among Sudanese Women in Khartoum State (2016-2020). |
Alkhair Abd Almahmoud Idris, P.hD Ahmed Abdelbadie Mohamed Suliman, MD , Elsadig Ahmed Adam, MD , Nazik Almalika Obeid
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Abstract:
Breast cancer is the commonest of human female cancers worldwide. This study aimed to evaluate the role of Cytocenterifugation Liquid Based Cytology (CLBC) in improving the efficacy of FNA in the detection of cytological changes in breast lumps among Sudanese women in Khartoum State. Material and Methods: This prospective cross-sectional study was carried out in Khartoum state (Sudan) among Sudanese women who suffered from breast lumps during August 2016- August 2020. FNA samples were collected from each patient, and the material was simultaneously smeared onto two slides and the remaining materials in the needle immersed in washing solution. Dry smears were stained with MGG stain, and wet fixed smears were stained with PAP stain. The remaining materials for CLBC were flushed out in a suspending solution. Then, two smears were prepared from each specimen, the fixed smears stained with Pap, and the dried smears were stained with MGG. For the evaluating the two stains PAP and MGG by CLBC and direct smears, scores were given on four parameters; the background of smears, the overall staining pattern, the cell morphology and the nuclear staining. The quality index was calculated as the ratio of the score achieved to the maximum possible score. Results: The findings revealed that the comparison between PAP and MGG direct smears (Conventional cytology) and CLBC smears, revealed that Pap stain CLBC smears stains were the best with a quality index of (0.80) and scored higher in all four parameters considered in the assessment. Ranked second was MGG CLBC smears stains with a quality index of (0.77). Ranked third was MGG direct smears with a quality index of (0.74). PAP stain direct smears ranked fourth with a quality index of (0.73). Conclusion: CLBC smears were better in staining than Conventional cytology smears in PAP and MGG stained slides.
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