Abstract:
This is hospital based descriptive retrospective case study aimed to detect the expression of laminin in breast tumors using immunohistochemical method. Forty paraffin embedded blocks from patients samples previously diagnosed as breast tumors were collected. One section of 3 micron thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method for detection of laminin. Data collected from patient's files and results were analyzed using SPSS computer program. Samples included 20 malignant tumors, including invasive ductal carcinoma 16 samples, lobular carcinoma 2 samples, micro papillary carcinoma one sample and metaplastic squamous cell carcinoma one sample. And 20 samples were benign tumors; all of them were fibroadenoma. Grade of malignant tumors were one sample was grade 1, 9 samples were grade II, 6 samples were grade III, 4 samples were not graded. The patients age range between 18 to 90 years with mean age of 40 years. Malignant breast tumors showed strong positive expression of laminin in 7 samples, and weak positive in 13 samples, while all benign tumors showed strong positive expression of laminin with significant association (P=0.000). This study concluded that breast tumor tissue express laminin with high expression in benign tumors.
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