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| Immunohistochemical Detection of Cytokeratin 20 in Sudanese Patients with Colorectal Carcinoma |
Salma Osman Mohamed , Abu Elgasim Abass Awad, Rayan Hassan Mohamed, Abuagla M. Dafalla
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Abstract:
Background: Colon cancer is cancer of the large intestine at the lower part of digestive system. Rectal cancer is cancer of the last several inches of the colon. Together, they are often referred to as colorectal cancers .The most common cancers of the large intestine (the type called adenocarcinoma) arise from the mucosa, the inner layer of cells. Mistakes (usually a series of mistakes involving genes within the replacement cells) lead to abnormal cells and uncontrolled proliferation of the abnormal cells that give rise to cancer. Objective: To detect the expression of cytokeratin 20 among colorectal carcinoma patients using immunohistochemical method, to correlate between CK20 expression and grade of cancer and to detect the association between colorectal cancer and age, sex of patients. Materials and methods: This is a hospital based cross-sectional retrospective descriptive study was conducted in Khartoum state during the period from May 2018 to July 2021. Thirty paraffin block samples were collected from patients previously diagnosed as colorectal carcinoma in National public health laboratory using simple random collection method. The paraffin blocks were cut by rotary microtome, and then stained by immunohistochemical method for detection of CK20. Results: The study revealed that the most patients were older than 50 years representing 56.7%. Out of thirty patients the study showed that the majority of patients were males representing 70%. CK20 among study population showed strong expression in 76.7% of patients, and weak expression in 23.3% of patients. The grade of cancer of study population revealed that 53.3% were well differentiated tumor. Conclusion: The study concludes that the CK20 expression is positive in all colorectal carcinoma tissue and the majority of expression is strong with no association with grade of cancer.
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