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| Assessment of the ability of extracts from Lawsonia inermis (Henna) as stains for cytology smears |
Eslam MohamedELfatih Ibrhim Suliman, Aisha Abdalgader Abdallah Albshir, Anfal Mohamed Elhassan Eldirdiri, Mariam Emad Eldeen Abdalla Mosaad, Mohammed Fathelrahaman Elamein, Nadin Abdalrheem Obaid Alhashmi, Sahar Abdel Rahman Zayed Adam Abuagla M. Dafalla,
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Abstract:
Background: Lawsonia inermis (henna) is a naturally occurring plant. The leaves of the henna plant are the source of red-brown dye widely known as a cosmetic agent. Mahalbiah and Serratia are famous henna oil. They- are consisting perfume, propylene glycol, geraniol and coumarin. Objective: The present study was to assess the henna ability in staining the cytoplasm and nucleus, color of henna in different extraction solvents, effect of henna on morphology of the cell, the effect of henna on nucleus with Mayer's and Haris's Hematoxylin and the effect of mahalbiah and serratia with henna in different extraction. Materials and Methods: The study was conducted at Wad Medani College of Medical Sciences and Technology, Medical Laboratory Science, Department of Histopathology and Cytology. This study was carried during December 2019 to December 2020. 41 Buccal smears stained by using hematoxylin and eosin method for cytological staining and replacing the eosin by the henna solutions (for 20 minutes) and added Mahalbiah and Serratia in method as step in stain for 10 minutes one time before solution (third one without mordant) and one time after solution. The data was analyzed using Scoring analysis by blinded person Does not have a background about the natural dyes which we used in the research.Results: The extraction of henna with absolute ethanol for cytoplasmic stain had been prevented by Harris's hematoxylin and gave blue cytoplasmic color, but in case of Mayer's hematoxylin gave dark brown cytoplasmic color. The extraction of henna didn't affect in nucleus stain with different type of hematoxylin (Mayer's and Harris's) and gave different score with different additives and different ways to apply the additives, the highest score recorded when Serratia applied before henna in stain. The extraction of henna with different additives didn't affect in nucleus stain with Mayer's hematoxylin.. The extraction of henna with normal saline for cytoplasmatic stain gave different score with different additives and different ways to apply the additives , the same extracted solution gave different result for nucleus and cytoplasm which improved with time. Henna extraction with normal saline, different additive and different way of apply them affected on score of nucleus with Mayer's hematoxylin. Henna with tap water only, and Mahalbiah with different ways of apply it gave the same score for cytoplasmatic stain, but in the case of using Serratia as additive with different ways of Applying it gave the same score for cytoplasm. Nucleus score with Mayer's hematoxylin gave different score with different additive. Conclusion: Henna with different additives (Mahalbiah and Serratia ) and extractions (ethanol absolute, normal saline, tap water) gave good score for cytoplasmic stain , but in absolute alcoholic extraction with serratia (applied before henna) gave the best score for cytoplasm and nucleus (stained with Mayer's hematoxylin) and didn't affect in cell morphology.
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