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| Association between Transcription Factor 7-like 2 (TCF7L2) rs7903146 gene polymorphism and Lipid profile among Diabetic Patients Refer to Abuagla Diabetic Center Gezira State, Wed Medani
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Rania Ali Abdalla Mohamed, Bakri Yousif Mohammed Noor, Shamseldein Mohmed Ahmed, Abuagla M. Dafalla, Adil Mergani , and Yousif abdelhameed Mohammed
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Abstract:
Type 2 diabetes mellitus (T2DM) is the most prevalent component of metabolic syndrome.. TCF7L2 plays a master role in regulating insulin biosynthesis, secretion, and processing .The main aim to associate of the rs7903146 single nucleotide polymorphism at the Transcription Factor 7-like 2 (TCF7L2) locus with type 2 diabetic patients. This is laboratory-based case control study conducted at Aboagalla Diabetic Center from 2017 to 2021. One hundred twenty participants, seventy T2DM patients as case and fifty as Non-Diabetic group were enrolled in this study. Five ml of blood were collected from each participant into (EDTA and heparin) anticoagulant tube to obtain plasma , lipid profile were measured by automated chemical analyzer (Cobse C311 Roche ) instrument also anthropometric (body weight, body height, and waist circumference were measured using the scale then DNA was extracted from whole blood using intron extraction kit and genotype of rs7903146 was carried out by a GeneAmp(r) PCR System 9700 (Applied Biosystems) thermo cycler .Genotype frequencies were examined for deviations from their corresponding Hardy-Weinberg equilibrium using SNP STAT software. SPSS software, version 22 was used for the rest of statistical analyses .Analysis of the distribution of the TCF7L2 rs7903146 genotype revealed that the GA genotype was more frequent in T2DM group (47.0%) than in Non-Diabetic (30%) (OR = 0.46, 95% confidence interval (CI = 0.21-0.99,p= 0.045). The T allele was more frequent in diabetic patients (48%) than control (41%). there is no statistically significant difference between the three genotypes regarding any of studied parameters except WC in the patient group was showed significant differences (p=0.035). It is concluded that the heterozygous genotypes GA, frequency of A alleles, dominant and over-dominant models of the rs7903146 of TCF7L2 gene are associated with the susceptibility of T2DM in the Sudanese population. We recommended studying all other SNPs of theTCF7L2 gene in our population which can potentially reveal the presence of other links with the susceptibility of developing diabetes and their complications.
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