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| Molecular Mutations in pfmdr1 Gene Codons ( 86, 184 and 1034) of Plasmodium falciparum related to Resistance to different Anti-malarial Drugs in Children attending to Pediatrics Teaching Hospital, Gezira State, Sudan
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Sana Ibrahim, Mohamed MY, Yousif Abdelhameed Mohammed, Bakri Yousif M. Nour, Abuagla M. Dafalla and Salaheldein G. Elzaki
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Abstract:
Malaria is still one of most problem that threating life in Sudan. Depending on the annual report for 2018, there were 1,617,499 true positive cases and 5.003 death. P.falciparum is the most prevalent species in Sudan with percentage of 91.2%.P.fMDR1which is located in chromosome 5 is still not clearly understudy regarding its role in drug resistance. Tis study aimed to detect and analyze three codons in this gene (codon 86,184, and 1034) and correlate the mutation to some drugs used in Gezira state, Sudan. Sample wre140 dried blood spot (DBS) which taken for molecular analysis (Nested PCR and RFLP).The study found that the percentage of mutation in the gene was 20.7% ( it was 6.4 %in codon 86,8.6%in codon 184 and 5.7% in codon 1034). There was no significant correlation between last treatment and mutation in the codons ,yet participant who Quartum therapy as last treatment ,had the highest percentage of mutation. The study revealed that PfMDR1shoud be concerned in a much attention that by doing massive scanning for it in endemic area with high observed drug failure. Also it revealed the drug policies needed to be revised again in this endemic area.
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