Title: Evaluation Of Antimicrobial Activity Of Fabricated Silver Nitrate Particles Obtained From Soursop Seeds Against Isolated Indoor Bacteria
Authors: EZEMBA, C.C., Victor, O. E., Ubah, B.O., Osuala, O.J., EZEMBA, A.S., Obiasogu, P.S., Mmaduekwe, J.A., Udoye, I. W., Ifeanyi-ofoka, C., Ezemba, P.I. and Eseigbu, A.C.
Volume: 8
Issue: 10
Pages: 35-45
Publication Date: 2024/10/28
Abstract:
Background: The antimicrobial potential of silver nitrate particles derived from soursop seeds against indoor bacteria was investigated in the Microbiology Departmental Laboratory, Uli, Anambra State, Nigeria. The study aimed to prepare and analyze the phytoparticle constituents of soursop seed extracts and evaluate the antibacterial efficacy of fabricated silver nanoparticles (Ag-NPs) against isolated indoor bacteria. Methodology: An extraction method was employed to obtain silver nanoparticles from soursop seeds, followed by antimicrobial assays using different concentrations of Ag-NPs and ciprofloxacin The setup was allowed to stand for 6-12 hr for diffusion to take place after which the plates were inverted and incubated at room temperature for 24 hr. After 24 hr, zones of inhibition were measured from two different angles and recorded. Result: The finding revealed that Isolate E (Pseudomonas, vibrio) was the most susceptible (27.5 ± 7.5) to the silver nitrate particles from Annona muricata seeds at 30mg/ml, while Isolate B (Aeromonas) had the lowest susceptibility (16.5 ± 0.5) to the extract at concentration of 30mg/ml. The results indicated significant susceptibility of indoor bacteria to Ag-NPs, with Pseudomonas spp. being the most susceptible. Moreover, Ag-NPs exhibited higher antimicrobial activity compared to ciprofloxacin Conclusion: This study has elaborately demonstrated the antimicrobial activity of silver nanoparticles extracted from Annona muricata seeds against isolated indoor bacteria. Therefore it is necessary to utilize the deduction from this research in developing novel therapies to combat multi-drug resistant organisms.