Title: Lead Acetate Induced Hepatotoxicity and Attenuation by Trigonella foenum-graecum and Curcuma longa in Male Albino Rats
Authors: Munira Ammar Al Gradi
Volume: 9
Issue: 1
Pages: 29-41
Publication Date: 2025/01/28
Abstract:
Background: Lead is widely used in many applications and so the potential to expose to its toxicity (animals and human) and environmental contamination is easier than other heavy metals. The exposure to lead possesses the potentials to induce hazardous biological effects in both animals and human beings. The antioxidants are important species that possess the ability to protect the body from damage caused by free radicals induced oxidative stress. Natural antioxidants strengthen the endogenous antioxidants defenses and restore the optimal balance by neutralizing reactive species. Objectives: The current study aimed to evaluate the alterations of liver function and histological structure of the liver induced by lead acetate in male albino rats and assess the protective role of natural materials (Trigonella foenum-graecum and Curcuma longa) against these alterations. Materials and Methods: Forty male albino rats (Rattus rattus) weighing between 150 and 210 grams were used in this study. Following a week of acclimatization, the animals were randomly assigned to five groups, each consisting of ten male albino rats: Group I (control group) was fed a regular diet and given tape water. Group II (group treated with lead acetate): For 30 days, the animals were fed 500 mg of lead acetate per kilogram of body weight. Group III (lead acetate/Trigonella foenumgraecum L seeds co-administered): For 30 days, the animals were given 500 mg of lead acetate per kilogram of feed along with 7.5 g of Trigonella foenumgraecum L seeds per kilogram of diet. Group IV (lead acetate/Curcuma longa): For 30 days, the animals were given 500 mg of lead acetate per kilogram of feed along with 20 g of Curcuma longa per kilogram of diet. Group V (lead acetate/Trigonella foenumgraecum L seeds + Curcuma longa co-administered): For 30 days, the animals were given 500 mg of lead acetate per kilogram of chow along with 7.5 g/kg of Trigonella foenumgraecum L seeds and 20 g/kg of Curcuma longa.. At the end of the experiment and 24 hours following the final dosage all animals were given ether anesthesia, and cardiac punctures were used to obtain blood samples for estimating the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT).