Title: Bioactive Properties of Castanea mollissima Leaf Extracts: Anti-Inflammatory, Antioxidant and Cytotoxic Activities
Authors: Truc Dang Huynh Dong Thuy Do Thi
Volume: 9
Issue: 11
Pages: 11-17
Publication Date: 2025/11/28
Abstract:
. This study evaluated the antioxidative, anti-inflammatory and cytotoxic activities of Castanea mollissima leaf extract collected in Cao Bang - Viet Nam , determining the most bioactive fractions for sequential isolation. Anti-inflammatory activity was assessed by measuring the inhibition LPS-induced NO production in RAW264.7 macrophages, antioxidant capacity assessed using DPPH free radical scavenging method and SC?? estimation, and anticancer activity indicated by MTT cytotoxicity estimation against the KB, HepG2, A549, and MCF-7 cell lines at IC? concentrations. The findings illustrate that every fraction apart from TKM repressed NO production with minimal cytotoxicity, while TKE had the strongest effect (IC?? = 18.5 µg/mL) followed by TK (20.2 µg/mL) and TKH (50.8 µg/mL). TKE also had the strongest results in the DPPH assay again outperforming the other fractions (SC = 76.28 ± 0.6%; SC?? = 20.65 µg/mL) while TK and TKH had moderate activities (SC?? = 36.12 and 51.35 µg/mL, respectively) and TKM had no activity (SC?? > 100 µg/mL). The cytotoxicity exhibited by the crude extract was the strongest against the A549 cancer line (IC?? = 8.96 ± 1.83 µg/mL), followed by KB (10.58 ± 1.66 µg/mL), HepG2 (11.02 ± 1.51 µg/mL), and MCF-7 (15.12 ± 1.94 µg/mL). Castanea mollissima leaves have anti-inflammatory, antioxidant and anti-cancer properties simultaneously with the active constituents concentrated in the ethyl acetate and n-hexane fractions. Future research should be directed towards the mechanistic study of Castanea mollissima leaves extract, the NO/NF-?B and oxidative stress pathways, and the in vivo evaluation of its safety and efficacy to support its pharmaceutical or cosmetic usage.