Title: Optimization of Extraction Conditions for Polysaccharides and Tannins from Ganoderma lucidum in Viet Nam
Authors: Lam Cao Thanh Ha
Volume: 9
Issue: 8
Pages: 217-221
Publication Date: 2025/08/28
Abstract:
Ganoderma lucidum (G. lucidum) is a medicinal mushroom famous for its health benefits and large amounts of bioactive substances like polysaccharides and tannins. The goal of this study was to determine the extraction optimization parameters for these two valuable compounds from the fruiting bodies of G. lucidum. The study examined systematically the effect of extraction method selected from decoction, maceration, ultrasonication, as well as, solvent type, material-solvent ratio, temperature, and time. For polysaccharide extraction, a Box-Behnken design and Response Surface Methodology (RSM) were employed to optimize the process. The results indicated that decoction was the most effective method for both polysaccharides and tannins. The optimal single-factor conditions for polysaccharide extraction were a material-to-solvent ratio of 1:50 (g/ml), a temperature of 100°C, and a time of 75 minutes, yielding 11.78 pm 0.06 mg/g. For tannin extraction, the optimal conditions were a water:ethanol ratio of 1:4 (v/v), a material-to-solvent ratio of 2:50 (g/ml), a temperature of 60°C, and a time of 75 minutes, resulting in a yield of 7.02 /pm 0.08 mg/g. RSM predicted a maximum yield of 12.80 mg/g of polysaccharide under the conditions of 1:43 material-to-solvent ratio, 100°C temperature, and 75 minutes extraction time. The study systematically optimized the extraction of the bioactive compounds from G. lucidum in order to provide a basis for their use in functional foods and pharmaceuticals. These optimized protocols show how bioactive polysaccharides and tannins can be efficiently recovered on a large scale. This is a good starting point for making functional foods, nutraceuticals, and pharmaceutical preparations from G. lucidum.