Title: Analysis and Evaluation of Antidiabetic Related Activities (PTP1B, ?-Glucosidase, Glucose Uptake) of Erythrina Variegata L. Extracts
Authors: Tran Tan Minh Khoi Nguyen Phi Hung
Volume: 9
Issue: 9
Pages: 79-88
Publication Date: 2025/09/28
Abstract:
Diabetes mellitus type 2 (T2DM) is one of the major health issues of our time, while Erythrina variegata L. (in Vietnamese, Vong nem) is a plant of considerable therapeutic promising potential. This study aims to assess optimized, particularly enriched extract from E. variegata for its multi-target antidiabetic potential. A refined method of extraction and enrichment was developed and applied. Three in vitro assays were performed: inhibition of protein tyrosine phosphatase 1B (PTP1B), inhibition of ?-glucosidase, and glucose uptake assays using 2-NBDG in 3T3-L1 adipocytes. The enrichment process greatly improved the biological activities of the extracts. The enriched extract E. variegata possessed an even more potent inhibition of both PTP1B (IC50 = 39.3 ± 1.7 µg/mL) and ?-glucosidase (IC50 = 78.4 ± 1.1 µg/mL) compared to the crude extract as well as the positive control Acarbose (IC50 = 123.5 ± 1.0 µg/mL). Moreover, the enriched extract also enhanced glucose uptake (Relative Fluorescence Unit - RFU = 82,655 ± 2,113), which is comparable to the positive control Ursolic Acid (RFU = 81,473 ± 1,720). The enriched extract of E. variegata showcases a potent, multi-targeted antidiabetic action in vitro which invites additional analysis as a phytomedicinal candidate for the treatment of T2DM.